Gametic Manipulation Bio-Technologies (TECNOGAM)

Strategic Area:
Introduction:

The research activity of the group Gametic Manipulation Bio-Technologies (TECNOGAM), recognized by the Government of Aragon as a consolidated Group of applied research, focuses on the implementation of various reproductive technologies for reproductive efficiency, the reduction of the health risk of transmission of diseases and the conservation of the animal biodiversity in mammals. The multidisciplinary team carries out work with a dual orientation, basic and applied, although both are interrelated.

Research Lines:
  • Topic 1: Epidemiological study of factors affecting reproductive efficiency in ruminants

    Reproductive efficiency is a key factor in the profitability of a farm. In this regard, we study the influence of different factors on reproductive efficiency in dairy cows and sheep (nutrition, environment, productive status, management, pathologies, etc.) and the interactions between them. To do this we carry out extensive epidemiological studies on the factors affecting various indicators of reproductive efficiency. We have analyzed factors related to the animal, management and environmental conditions. The results have improved the reproductive efficiency of dairy farms.

  • Topic 2: Artificial insemination: basic and applied aspects.

    For a successful artificial insemination (AI) is necessary to make a correct oestrus detection or synchronization, and to deposit, at an adequate time and place, good quality seminal dose in genital tract of a female in good condition. Our goal is related to several factors influencing fertility applying the technique of artificial insemination. In particular we have worked on methods of detection and synchronization of oestrus prior to the IA and the importance of the optimum insemination time and the place of semen deposition. We have also studied sperm transport in the female genital tract after AI. Techniques of optical and electronic microscopy and studies on the physical properties of the cervical mucus and its relationship with sperm transport and fertility have been used for this. Some of the techniques developed are applied for farming.

  • Topic 3: Other reproductive biotechnologies

    The overall objective of this line is the improvement and application of biotechnologies related to gametes and embryos in domestic mammals. These include the development of media for the embryo culture and semen preservation, the improvement of protocols of freezing and vitrification of oocytes and embryos and the development and improvement of techniques sperm quality analysis and its relationship to field fertility.

Background:

The Group presents an intense scientific activity, with the publication of more than 50 SCI-papers in the last 5 years and the development of more than 30 research regional, national and international projects. It also maintains active collaboration with groups of researchers from Spain and other countries. The experience of the Group focuses mainly on the study of the reproductive efficiency of ruminants and biotechnologies that contribute to its improvement. For this we carry out basic and applied studies to help us understand the causes of reproductive inefficiency and strategies to overcome them. We are experts in epidemiological studies related to reproduction, in the improvement of the protocols for detection and synchronization of oestrus, semen quality assessment and preservation, in the technique of artificial insemination and in vitro production of embryos.

Facilities and equipment:

Techniques available

  • Microscopy laboratory: Fluorescence microscopy, Brightfield, Phase contrast, DIC, Hoffman etc.
  • Imageanalisys.
  • SpermCASA-system
  • Equipment for gamete micromanipulation
  • ELISA
  • FIV-laboratory
  • Molecular biologylaboratory.

METODOLOGY

  • Oestrusdetection and synchronization

    • Advancedoestrusdetection
    • New methods of oestrussynchronization
  • Semen qualityassessment

    • CASA sperm motility
    • ASMA sperm morphometry
    • Sperm viability
    • Sperm DNA fragmentation (TUNEL, SCD)
    • Mitocondrial activity
    • Zona binding assay (ZBA), In vitro penetration test (IPT)
    • FIV
  • Sperm partitioning

    • Swim-up
    • Gradients: Percoll
  • Semen preservation

    • New semen diluents
    • Semen cryopreservation
  • Artificial insemination (AI)

    • Cervical AI
    • Intrauterine AI
    • Intraperitoneal AI
  • Molecular biology

    • PCR
    • Electrophoresis
    • Western-Blot
    • FISH
  • Embryoproduction and recovery

    • MOET programs
    • Ecography
    • Oocyte-embryocryopreservation
  • FIV

    • IVM, IVF, IVC
Technological Offer/Services:
  • Advice about reproductive management of farms
  • Advice on methods of synchronization of oestrus and artificial insemination
  • Advice on implementation of reproductive biotechnologies
  • Optical and electronic microscopy (SEM) scan or transmission (TEM) studies
  • Semen quality studies (spermiogram).
  • Semen extenders development
Contact:

Jesús Yániz
Head of group
jyaniz@unizar.es

Dept. Animal Production and Food Science

Technological College – Huesca

Other members